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DNA01
DNA Test Sample
DNA01 is Plasmid pGem7zf+ (Promega), which is linearized with the SmaI endonuclease. Linear DNA molecules (3000 b. p.) are deposited at the freshly cleaved mica. Molecules are uniformly distributed over the surface with the molecular density - 0,5-7 molec./um2. The typical DNA length is 1009 nm. Recommended humidity for obtaining a good image is 3-5%.
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| Typical AFM image of the DNA test sample (obtained in contact mode, humidity 1-10%, Solver P47BIO, NT-MDT Co.). |
Histogram indicating distribution of DNA length, mean value - 1009 nm, standard deviation - 27 nm. |
- Getting started with your work on AFM; - Example of how to prepare your own DNA samples; - Estimation of cantilever tip curvature; - Humidity test; - Z-resolution test.
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